1. Identification results of Astral DIA using different sample preparation strategies
2. I performed Western Blot (WB) validation later, but the trend was opposite to the proteomic results. What could be the reasons?
There are several potential explanations for this discrepancy:
Protein Modification Status: In some cases, a specific physiological or pathological phenomenon is not correlated with the total abundance of a protein, but rather with the abundance of its modified form.
Upstream Signaling Factors: Within a signaling pathway, the key regulatory proteins located upstream may not have been captured or detected in the initial analysis.
Peptide Quality and Quantification: It is also important to check the quality of the peptides corresponding to the protein of interest. Low peptide quality can lead to inaccurate quantification in the proteomic data.
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