The PacBio Revio long-read system leverages Single Molecule, Real-Time (SMRT) sequencing technology powered by Zero-Mode Waveguides (ZMWs). A laser illuminates the bottom of each ZMW, where a DNA polymerase is anchored; as fluorescently labeled dNTPs are incorporated, they emit distinct signals that enable precise base identification. The system operates in Circular Consensus Sequencing (CCS) mode—generating HiFi reads by combining multiple subreads from the same DNA molecule into a single consensus sequence. This delivers exceptional accuracy with quality values exceeding Q30 (>99.9%).
Workflow
Highlights
lLong Reads
Read lengths of over tens of kilobases (kb), with the longest exceeding 100 kb
Capable of sequencing insert sizes ranging from 500 bp to over 50 kb
lHigh Accuracy
No systematic error
When the sequencing depth reaches 30X, the accuracy can reach >99.999% (Q50)
lSingle-Molecule Resolution
Q30 (99.9%) long-read single-molecule accuracy
lUniform Coverage
No preference for GC content or sequence complexity
lSimultaneous Epigenetic Detection
Application Areas
Genome Assembly
Microbial Metagenomics
Full-Length Transcriptomics
Epigenome Sequencing
Targeted Detection
Tumor Gene Testing
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