HiFi reads combine long-read length with high accuracy, replacing multiple assays and confidently resolving complex regions beyond the limits of short reads and nanopore methods.
Built for population-scale studies and programs that don’t pause. Run up to ~2,500 human whole genomes per year, up to 480 Gb of HiFi reads per day, and up to 4 SMRT Cells simultaneously with configurable run times and flexible scheduling — so sequencing keeps pace with your science.
Every standard run delivers on-instrument 5mC and 6mA methylation calling—at no additional cost or bisulfite treatment—giving you access to genomic and epigenetic data from a single sample.
HiFi accuracy powered by SPRQ chemistry and Google Health DeepConsensus, paired with an easy, automation-ready workflow supported by compatible partners—so you get reliable results with minimal hands-on time.
The Revio system supports a variety of applications that benefit from accurate long HiFi reads and 500 ng input DNA requirement. Its four independent stages allow different samples and applications to be sequenced in parallel. A subset of key applications is:
The Revio system with SPRQ chemistry produces exceptional read length, quality, and variant calling performance that have made HiFi sequencing so celebrated.
The complete, gapless human genome marks a new era of genomics where no region of the genome is beyond your reach. In 2021, PacBio HiFi technology was used by the Telomere-to- Telomere (T2T) Consortium to fill in those gaps.
HiFi reads are tens of kilobases long, providing the ability to resolve large variants and map to difficult regions of the genome.
The Revio system provides reliable answers through exceptional accuracy, with 90% of bases Q30+ and median read accuracy ≥Q30.
HiFi sequencing with SPRQ chemistry on the Revio system delivers high accuracy variant detection, resulting in fewer sequencing errors relative to other technologies at lower coverage.
The Revio system simplifies the HiFi sequencing experience with fewer consumables optimized for easy tracking and minimal waste. With workflow improvements, loading consumables takes under a minute at the instrument.
One tray contains 4 high-density SMRT Cells.
The sequencing plate provides reagents for sequencing up to 4 Revio SMRT Cells.
The Revio system gives you a vastly simplified experience with consumables and software designed for flexibility and convenience. Whether you’re a genome center or a small lab, you can enjoy:
Library prep for the Revio system supports manual and automated prep.
Sequencing plates are linked automatically to run designs through an NFC tag, enabling runs to be set up in less than one minute on instrument.
The Revio work deck is isolated from the four sequencing stages, leaving the system available up to 20 hours per day for loading consumables for subsequent runs.
The Revio system utilizes nanofabricated Revio SMRT Cells and has four independent stages, allowing sequencing of multiple SMRT Cells in parallel. The onboard compute provides accurate basecalling with Google DeepConsensus, plus methylation calling in every run.
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