Instruments & Reagents

Introduction

The Rh blood group system is the most complex and highly polymorphic of all human erythrocyte antigen systems, with clinical significance second only to the ABO system. Precise genotyping of this system constitutes a core technical requirement across transfusion medicine, medical genetics, organ transplantation, and forensic science. Moreover, it serves as a critical prerequisite for implementing precision transfusion protocols and establishing robust safeguards for patient safety.

The HemoSure™ Long-Read Sequencing Rh Blood Group Genotyping Kit (Single-Molecule Real-Time Sequencing)  leverages cutting-edge sequencing technology to deliver a high-resolution genotyping solution, providing professional and reliable auxiliary support for clinical Rh blood typing.

Intended Purpose

This kit is a qualitative genotyping assay based on polymerase chain reaction (PCR) and PacBio HiFi sequencing platforms, designed for identifying the allele types of the RHD, RHCE and RHAG gene in genomic DNA extracted from EDTA-collected whole blood specimens. This test can detect polymorphisms of the Rh blood group, determine allelic genotypes, and predict corresponding antigen phenotypes, serving as a viable alternative to serological testing.

The workflow utilizes polymerase chain reaction (PCR) to amplify a predefined set of target genes. The resulting amplicons are designed for downstream library preparation and sequencing on PacBio HiFi platforms. The generated sequencing data is subsequently analyzed using Haorui TransMatch^TM software to produce high-resolution Rh genotyping results.

Note: Test results obtained with this kit are for clinical reference only and should not be used as the sole basis for diagnosis or clinical decision-making. Clinicians should make comprehensive judgments and treatment decisions by integrating these results with the patient’s medical history, physical examination, and other laboratory findings.

Principle of the Method

HemoSure^TM Long-Read Sequencing Rh Blood Group Genotyping Kit serves as an integrated solution for amplifying target genes of the Rh blood group system and preparing sequencing libraries. The kit facilitates the amplification of RHD, RHCE, and RHAG genes, followed by library preparation for downstream sequencing.

(1) Nucleic Acid Extraction: Isolate DNA from samples.

(2) PCR Enrichment: Amplify target genes (RHD, RHCE, and RHAG).

(3) Library Prep: Ligate PacBio’s proprietary SMRTbell adapters to construct circular DNA libraries.

(4) Sequencing: Load libraries onto Revio’s or Vega’s long-read sequencing platform with HiFi sequencing mode.

(5) Fluorescently labeled nucleotides are incorporated during polymerization, and real-time signals are captured to generate HiFi reads.

(6) Data Analysis: Align reads to the human genome and RHD, RHCE, and RHAG reference sequences (International Society of Blood Transfusion Database) and export the Rh genotyping results.

Product Configuration

Shipping and Storage

1. Storage Conditions

Store the kit at -20 ±5°C. The shelf life is 3 months from the date of manufacture (Note: Magnetic beads should be stored in a 4°C refrigerator).

2. Freeze-Thaw Stability

The kit may undergo no more than 6 freeze-thaw cycles. Once opened, use immediately.

3.  Shipping Requirements

Transport under temperature-controlled conditions (-20 ±5°C).

Maximum allowable transit time: 3 days.

Upon receipt, verify that the internal temperature remains within -20 ±5°C before opening.

4. Refer to the lot-specific label for manufacturing date (MFG) and expiration date (EXP).

For general inquiries, orders, pricing, delivery, or product usage information, please reach out to  us via email: marketing@xahorizon.cn.