Introduction

Unlock the full potential of precision medicine with our latest genotyping solution. Designed for transfusion medicine, transplantation, and forensic applications, the HemoSure™ Long-Read Sequencing RBC Blood Groups Genotyping Kit (Panel 2) delivers the accuracy required for critical clinical diagnostics.

Key Features & Benefits:

• Powered by PacBio HiFi: Utilizes state-of-the-art long-read sequencing for superior resolution of complex genomic regions.

• Comprehensive Coverage: A single assay covering 9 major blood group systems (ABO, Rh, MNS, Kidd, Duffy, P1PK, Kell, Lewis, Diego).

• Targeted Precision: Deep analysis of 13 essential genes (ABO, RHD, RHCE, GYPA, GYPB, SLC14A1, ACKR1, A4GALT, B3GALANT1, KEL, FUT2, FUT3, SLC4A1 ).

• Clinical Confidence: Provides robust data to support precise blood typing and enhance patient safety.

Elevate your laboratory's capabilities with a reliable, high-throughput solution for red blood cell genotyping.

Intended Purpose

 The kit provides a streamlined workflow for the simultaneous detection of variants in the ABO, RHD, RHCE, GYPA, GYPB, SLC14A1, ACKR1, A4GALT, B3GALANT1, KEL, FUT2, FUT3, and SLC4A1 genes. Covering the ABO, Rh, MNS, Kidd, Duffy, P1PK, Kell, Lewis, Diego blood group systems, it serves as a vital auxiliary diagnostic tool for implementing precision transfusion strategies and ensuring clinical safety in transfusion medicine, genetics, and transplantation fields.

Note: Test results obtained with this kit are for clinical reference only and should not be used as the sole basis for diagnosis or clinical decision-making. Clinicians should make comprehensive judgments and treatment decisions by integrating these results with the patient’s medical history, physical examination, and other laboratory findings.

Principle of the Method

HemoSure™ Long-Read Sequencing RBC Blood Groups Genotyping Kit (Panel 2) (Single-Molecule Real-Time Sequencing) serves as an integrated solution for amplifying target genes of the ABO, Rh, MNS, Kidd, Duffy, P1PK, Kell, Lewis, Diego blood group system and preparing sequencing libraries. The kit facilitates the amplification of ABO, RHD, RHCE, GYPA, GYPB, SLC14A1, ACKR1, A4GALT, B3GALANT1, KEL, FUT2, FUT3, and SLC4A1 genes, followed by library preparation for downstream sequencing.

(1) Nucleic Acid Extraction: Isolate DNA from samples.

(2) PCR Enrichment: Amplify target genes (ABO, RHD, RHCE, GYPA, GYPB, SLC14A1, ACKR1, A4GALT, B3GALANT1, KEL, FUT2, FUT3, and SLC4A1).

(3) Library Prep: Ligate PacBio’s proprietary SMRTbell adapters to construct circular DNA libraries.

(4) Sequencing: Load libraries onto Revio’s or Vega’s long-read sequencing platform with HiFi sequencing mode.

(5) Fluorescently labeled nucleotides are incorporated during polymerization, and real-time signals are captured to generate HiFi reads.

(6) Data Analysis: Align reads to the human genome and ABO, RHD, RHCE, GYPA, GYPB, SLC14A1, ACKR1, A4GALT, B3GALANT1, KEL, FUT2, FUT3, and SLC4A1 reference sequences (International Society of Blood Transfusion Database) and export the blood group genotyping results.

Product Configuration

Shipping and Storage

1. Storage Conditions

Store the kit at -20 ±5°C. The shelf life is 3 months from the date of manufacture (Note: Magnetic beads should be stored in a 4°C refrigerator).

2. Freeze-Thaw Stability

The kit may undergo no more than 6 freeze-thaw cycles. Once opened, use immediately.

3.  Shipping Requirements

Transport under temperature-controlled conditions (-20 ±5°C).

Maximum allowable transit time: 3 days.

Upon receipt, verify that the internal temperature remains within -20 ±5°C before opening.

4. Refer to the lot-specific label for manufacturing date (MFG) and expiration date (EXP).

For general inquiries, orders, pricing, delivery, or product usage information, please reach out to  us via email: marketing@xahorizon.cn.